Delta & Pine Patent for Control of Plant Gene

Delta and Pine- Patent for Control of Plant Gene Expression, March 1998

5723765 : Control of plant gene expression

INVENTORS:

Oliver; Melvin John, Lubbock, TX
Quisenberry; Jerry Edwin, Idalou, TX
Trolinder; Norma Lee Glover, Quanah, TX
Keim; Don Lee, Leland, MS

ASSIGNEES:

Delta and Pine Land Co., Scott, MS

The United States of America as represented by the Secretary of Agriculture, Washington, DC

ISSUED:

Mar. 3 , 1998

FILED:

June 7 , 1995

SERIAL NUMBER:

477559

MAINT. STATUS:

INTL. CLASS

(Ed. 6):

C12N 015/29; C12N 015/82; A01H 004/00; A01H 005/00;

U.S. CLASS:

800/205; 800/250; 536/024.1; 536/023.6; 536/024.5; 435/320.1; 435/240.4; 435/172.3;

FIELD OF SEARCH:

536-24.1,23.6,24.5 ; 435-320.1,240.4,172.3 ; 800-205,250 ;

ABSTRACT: A method for making a genetically modified plant comprising regenerating a whole plant from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked on either side by specific excision sequences, a second gene that encodes a recombinase specific for the specific excision sequences linked to a repressible promoter, and a third gene that encodes the repressor specific for the repressible promoter. Also a method for making a genetically modified hybrid plant by hybridizing a first plant regenerated from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked on either side by specific excision sequences to a second plant regenerated from a second plant cell that has been transfected with DNA sequences comprising a second gene that encodes a recombinase specific for the specific excision sequences linked to a promoter that is active during seed germination, and growing a hybrid plant from the hybrid seed. Plant cells, plant tissues, plant seed and whole plants containing the above DNA sequences are also claimed.

U.S. REFERENCES: (No patents reference this one)

Patent

Inventor

Issued

Title

4959317

Sauer

9 /1990

Site-specific recombination of DNA in eukaryotic cells

5159135

Umbeck

10 /1992

Genetic engineering of cotton plants and lines

5217889

Roninson et al.

6 /1993

Methods and applications for efficient genetic suppressor elements

5231019

Paszkowski et al.

7 /1993

Transformation of hereditary material of plants

5244802

Rangan

9 /1993

Regeneration of cotton

5270201

Richards et al.

12 /1993

Artificial chromosome vector

5304730

Lawson et al.

4 /1994

Virus resistant plants and method therefore

5352605

Fraley et al.

10 /1994

Chimeric genes for transforming plant cells using viral promoters

EXEMPLARY CLAIM(s): Show all 55 claims

We claim:
1. A method for making a genetically modified plant comprising

stably transforming a plant cell with a first DNA sequence comprising a first gene whose expression results in an altered plant phenotype, and a transiently-active promotor, the first gene and the transiently-active promotor being operably linked to one another, but separated by a blocking sequence that is flanked by specific excision sequences, such that the presence of the blocking sequence prevents the expression of the first gene, a second DNA sequence comprising a second gene that encodes a recombinase specific for the specific excision sequences flanking the blocking sequence of the first DNA sequence, and a repressible promotor operably linked in functional relation to the second gene, and a third DNA sequence comprising a third gene that encodes a repressor specific for the repressible promotor of the second DNA sequence, the third sequence being linked to a plant-active promoter;
regenerating a whole plant from the plant cell.

RELATED U.S. APPLICATIONS:

Patent No.

Appl No.

Issue Date

/details?patent_number=

283604

This is a continuation-in-part application of application Ser. No. 08/283,604, filed on Aug. 1, 1994, now abandoned.

FOREIGN APPLICATION PRIORITY DATA: none

FOREIGN REFERENCES:

Document No.

Country

Date

Intl. Class

131623

EPO

3 /1991

9008826

WIPO

8 /1990

9109957

WIPO

7 /1991

9403619

WIPO

2 /1994

OTHER REFERENCES:

Schena et al. A steroid-inducible gene expression system for plant cells. Proc. Natl. Acad. Sci. USA. vol. 88, pp. 10421-10425, Dec. 1991.
Gatz et al. Regulation of a modified CaMV 35s promoter by the TN10-encoded Tet repressor in transgenic tobacco. Mol. Gen. Genet. (1991) 227:229-237.
Bayley et al. Exchange of gene activity in transgenic plants catalyzed by the Cre-lox site-specific recombination system. Plant Molecular Biology 18: 353-361, 1992.
Galau et al. Cotton Lea4(D19) and LeaA2(D132) Group 1 Lea Genes Encoding Water Stress-Related Proteins Containing a 20-Amino Acid Motif. Plant Physiol. (1992) 99, 783-788.
Barthelemy et al. The Expression of Saporin, a Ribosome-inactivating Protein from the Plant Saponaria officinalis, in Escherichia coli. J. Biol. Chem. vol. 268, No. 9, p. 6546548, Mar. 1993.
Lanzer, Michael and Bujard, Hermann, "Promoters largely determine the efficiency of repressor action," Proc. Natl. Acad. Sci. USA, 85: 8973-8977 (1988).
Araki, Kimi et al., Site-specific recombination of a transgene in fertilized eggs by transient expression of Cre recombinase, Proc. Natl. Acad. Sci. USA, 92(1):160-164 (1995).
Medberry, Scott L., et al., Intra-chromosomal rearrangements generated by Cre-lox site-specific recombination, Nucleic Acids Research, 23(3):485-490 (1995).
Chapman, Sean, Kavanagh, Tony and Baulcombe, David, "Potato virus X as a vector for gene expression in plants," The Plant Journal, 2(4):549-557 (1992).
Odell, Joan T., et al., Seed-Specific Gene Activation Mediated by the Cre/lox Site-Specific Recombination System, Plant Physiol., 106:447-458 (Oct. 2, 1994).
Qin, Minmin, et al., Cre recombinase-mediated site-specific recombination between plant chromosomes, Proc. Natl. Acad. Sci. USA, 91:1706-1710 (1994).
Sauer, Brian, Manipulation of Transgenes by Site-Specific Recombination: Use of Cre Recombinase, Methods in Enzymology, 225:890-900 (1993).
Barinaga, Marcia, "Knockout mice: round two," Science, 265:26-28 (1994).
Gu, Hua, Marth, Jamey D. Orban, Paul C., Mossmann, Horst and Rajewsky, Klaus, "Deletion of a DNA of a polymerase B gene segment in T cells using cell type-specific gene targeting," Science, 265:103-106 (1994).
Geissendorfer and Hillen, "Regulated expression of heterologous genes in Bacillus subtilis using the Tn10 encoded tet regulatory elements," Appl. Microbiol. Biotechnol., 33:657-663 (1990).
Gatz and Quail, "Tn10-encoded tet repressor can regulate an operator-containing plant promoter," Proc. Natl. Acad. Sci. USA, 85:1394-1397 (1988).
Gatz, et al., "Regulation of a modified CaMV 35S promoter by the Tn10-encoded Tet repressor in transgenic tobacco," Mol. Gen. Genet., 227:229-237 (1991).
Gatz, et al., "Regulation of a plant promoter by a bacterial repressor protein in transgenic tobacco plants," (Abstract) Herbsttagung der Gesellschaft fur Biologische Chemie, 372:659-660 (1991).
Roder, et al., "Efficiency of the tetracycline-dependent gene expression system: complete suppression and efficient induction of the rolB phenotype in transgenic plants," Mol. Gen. Genet., 243:32-38 (1994).
Gatz, et al., "Stringent repression and homogenous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants," The Plant Journal, 2:397-404 (1992).
Rogers, S.G., Klee H.J., Horsch, R.B. and Fraley, R.T., "Improved vectors for plant transformation: expression cassette vectors and new selectable markers," Methods in Enzymology, 153:253-305 (1987).
Shen, Wen-Hui and Hohn, Barbara, "Excision of a transposable element from a viral vector introduced into maize plants by agroinfection," The Plant Journal, 2(1):35-42 (1992).
Pan, Guohua, Luetke, Karen and Sadowski Paul D., "Mechanism of cleavage and ligation by FLP recombinase: classification of mutations in FLP protein by in vitro complementation analysis," Molecular and Cellular Biology, 13(6):3167-3175 (1993).
Hall, Samantha C. and Halford, Stephen E., "Specificity of DNA recognition in the nucleotprotein complex for site-specific recombination by Tn21 resolvase," Nucleic Acids Research, 21(24):5712-5719 (1993).
Wilde, Robin J., Cooke, Susan E., Brammar, William J. and Schuch, Wolfgang, "Control of gene expression in plant cells using a 434:VP16 chimeric protein," Plant Molecular Biology, 24:381-388 (1994).
Muskhelishvili, Georgi, Palm, Peter and Zillig, Wolfram, "SSV1-encoded site-specific recombination system in Sulfolobus shibate," Mol. Gen Genet 237:334-342 (1993).
Sadowski, Paul D., "Site-specific genetic recombination: hops, flips, and flops," The FASEB Journal, 7:760-767 (1993).
Lloyd, Alan M., Davis, Ronald W., "Functional expression of the yeast FLP/FRT site-specific recombination system in Nicotiana tabacum," Mol. Gen. Genet., 242:653-657 (1994).
Horn, Mark A., Heinstein, Peter F. and Low, Philip S., "Biotin-mediated delivery of exogenous macromolecules into soybean cells," Plant Physiol., 93:1492-1496 (1990).
Mett, Vadim L., Lochhead, Leesa P. and Reynolds, Paul H.S., "Copper-controllable gene expression system for whole plants," Proc. Natl. Acad. Sci. USA, 90:4567-4571 (1993).
Schena, Mark, Lloyd, Alan M. and Davis, Ronald W., "A steroid-inducible gene expression system for plant cells," Proc. Natl. Acad. Sci. USA, 88:10421-10425 (1991).
Wilde, R.J., Shufflebottom, D., Cooke, S., Jasinska, I., Merryweather, A., Beri, R., Brammar, W.J., Bevan, M. and Schuch, W., "Control of gene expression in tobacco cells using a bacterial operator--repressor sysetm", The EMBO Journal, 11(4):1251-1259 (1992).
Hamamoto, Hiroshi, Sugiyama, Yoshinori, Nakagawa, Noriaki, Hashida, Eiji, Matsunaga, Yuji, Takemoto, Shizume, Watanabe, Yuichiro and Okada, Yoshimi, "A new tobacco mosaic virus vector and its use for the systemic production of angiotensin-I-converting enzyme inhibitor in transgenic tobacco and tomato," Biotechnology, 11:930-932 (1993).
Zou, Y.R., Muller, W., Raiewsky, K., Cre-loxP-mediated gene replacement: a mouse strain producing humanized antibodies. Current biology. Dec. 1, 1994 4(12):1099.

ATTORNEY, AGENT, or FIRM:	Rothwell, Figg, Ernst & Kurz;
PRIMARY/ASSISTANT EXAMINERS:	Robinson; Douglas W.; Haas; Thomas